Jacob Biltius or Jacobus Biltius was a Dutch still life painter originally from The Hague who worked in various places including The Hague, Amsterdam, Maastricht, Antwerp, Leeuwarden and Bergen op Zoom. He was known for his game still lifes, kitchen still lifes and trompe-l'œil still lifes.

Jacob Biltius was born in The Hague as the son of Bartholomeus Bilt and an unknown mother. The profession of his father is not known. He was baptized on 27 November 1633. He studied in The Hague under the obscure French painter Carel Hardy around 1651 and later with Pieter de Putter, a painter known for his fish still lifes. Biltius was admitted in 1660 to the Confrerie Pictura, which was founded in 1656 by local painters, who were unsatisfied by The Hague Guild of St Luke.

He married Adriana Proot van der Jeucht in 1653. The couple had 8 children of whom Cornelis and Bartholomeus became painters. In 1661, Biltius moved to Amsterdam, where he remained until 1666. Between 1666 and 1670 he was active in Maastricht.

In 1671 he returned for a few months to Amsterdam, but then moved to Flanders, settling in Antwerp with his family. In the same year Biltius and his wife joined the secret society of local Calvinists, 'de Consistorie van de Brabantsche Olijfberg' (Consistory of the Olive Mountain of Brabant). The Consistorie’s best known member was the Flemish painter Jacob Jordaens whose house was the venue where its members congregated between 1674 until his death in 1678. The Calvinists also gathered in Biltius’ Antwerp home on a few occasions. He was admitted to the Antwerp Guild of St Luke in the Guild year 1672-1673. He remained active in Antwerp until 1678.

Biltius later moved to Bergen op Zoom where he died in 1681.