Niccolò Giolfino was an Italian painter of the Renaissance period, active mainly in Verona.

He was a pupil of Liberale da Verona. One source attributes the influence of Andrea Mantegna. He painted mainly sacred subjects for local churches. He painted two canvases for a chapel in San Bernardino. He painted frescoes for the church of Santa Maria in Organo and in a chapel in San Bernardino. His brother, Paolo Giolfino, painted in a similar style. Another of his pupils was Paolo Farinato.