Girolamo da Santacroce was a 16th-century Italian painter of the Renaissance period, active mainly in Venice and the Venetian mainland.

Girolamo da Santacroce was born in Bergamo, Italy. He became in Venice a pupil of the painter Gentile Bellini. On Gentile's death in 1507, Santacroce was left in Gentile's will half of the Oriental drawings made be Gentile. Santacroce then most likely worked as an assistant in the workshops of Giovanni Bellini and Cima da Conegliano.

He was a prolific artist and many of his works are signed and dated. He produced many copies after the works of the leading Venetian masters. His work shows the influence of Titian and Palma Vecchio.