Johann Matthias Wurzer was an Austrian painter from Salzburg.

Wurzer came to Salzburg in 1776 at the age of 16, became an apprentice to the painter Franz Karl Zircher (1741–1793) and stayed with him until 1782.

The Gurk Prince-Bishop Franz II Xaver von Salm-Reifferscheidt-Krautheim , who employed Zircher, recognized the talent of the young Wurzer and sent him to the Academy of Fine Arts Vienna, where Wurzer learned flower and fruit painting from the flower painter Johann Baptist Drechsler. After nine years of study, Prince Salm called him to Klagenfurt, where he stayed for six to seven years, taking on the plants of the local and neighboring Alpine regions.

Wurzer returned to Salzburg around 1795, where he acquired citizenship in 1797. He married the gardener's daughter from Gnigl, Theresia Starchl.

He mainly painted still lifes of flowers and fruits as well as landscapes. He also gave painting lessons. His students included Georg Pezolt and Anton Reiffenstuhl.