Sebastiano Mazzoni was an Italian painter of the Baroque period.

Born in Florence, he trained in that city during 1632-33 in the studio of Baccio del Bianco. He then moved to Venice in 1648, and stayed there till his death. He painted a somewhat unusual Annunciation with a hovering ghostly angel dominating the scene. In 1638 he joined the Accademia del Disegno in Florence. Andrea Celesti was one of his pupils; and it is said he influenced the style of Sebastiano Ricci and Ghislandi. Ultimately he has an enigmatic individual style with paintings of unresolved dynamism, depicted from awkward perspectives, in some fashion he resembles his contemporary Francesco Maffei or evokes the distortions of a Lorenzo Lotto of the prior century.