Karl Biese was a German landscape painter, draughtsman and lithographer.

After his apprenticeship as a painter, he initially worked as a theater painter. In 1883 he was awarded a scholarship to the Karlsruhe Academy of Art. He returned to Hamburg in 1886, passed his master painter's examination and set up his own business. In 1892, he sold his painting business and returned to the Karlsruhe Academy. There he became a master student of Gustav Schönleber. In 1899, he and his wife moved to Grötzingen to the former Augustenburg Margravial Palace, where his fellow artists Jenny Fikentscher, Otto Fikentscher, Franz Hein and Gustav Kampmann from the Grötzingen painters' colony already lived. After numerous other moves, he lived in Tübingen from 1917.

Biese was a co-founder of the Karlsruher Künstlerbund and, after Franz Hein, its chairman for some time. The Karlsruhe Artists' Association had its own lithography workshop, the Kunstdruckerei Künstlerbund Karlsruhe.