Girolamo Macchietti was an Italian painter active in Florence, working in a Mannerist style.

He was a pupil of Michele di Ridolfo. During the years 1556-1562, worked as an assistant to Giorgio Vasari in the decoration of the Palazzo Vecchio, where he worked with Mirabello Cavalori. He participated in the Vasari-directed decoration of the Studiolo of Francesco I with two canvases, one relating a Jason and Medea (1570) and the other a Baths of Pozzuoli (1572). He also painted an altarpiece on the Martyrdom of Saint Lawrence for Santa Maria Novella. In 1577, he completed a Gloria di San Lorenzo for Empoli Cathedral. He traveled to Rome and spent two years in Spain (1587–1589). No works are recorded from these travels.